Système immunitaire et méthamphétamine

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Système immunitaire et méthamphétamine: base moléculaire d’une relation 

Use of methamphetamine (Meth) as a drug of abuse is on the rise worldwide. Besides its effect on the function of the brain, Meth has detrimental effects on how the immune system functions. As documented in the literature, various experimental models (cellular, animal, mice, and non-human primates) have been used that have contributed to the overall knowledge about immune system impairments from Meth exposure. It has to be noted that while Meth is used in very few treatments, it affects a broad range of biological mechanisms, not only immune regulation, in a negative manner.

Undoubtfully, the effect of Meth is highly complex; moreover, the initial molecular triggers remain unknown. Analyses of available literature suggests that the effect of Meth is not prompted by one underlying mechanism. Whether the effect of Meth is acute or long-lasting, the overall effect is negative. Further advancement of our knowledge on Meth’s specific actions will require systematic experimental approaches using all available models. In addition, bioinformatic analyses are necessary to build a comprehensive model as a needed tool to fill the gap in knowledge.

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Tris - Hydrochloride (Molecular Biology Grade)

CE235 500 g
EUR 144

Tris - Hydrochloride (Molecular Biology Grade)

CE236 1 kg
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Guanidine thiocyanate?For Molecular Biol

MB015-1KG 1 unit
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MB015-500G 1 unit
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09860-25 500G
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Guanidine Hydrochloride

17318-24 10KG
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Guanidine Hydrochloride

17318-82 25G
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17318-95 500G
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Guanidine Hydrochloride

17319-14 100G
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Guanidine Hydrochloride

17319-85 500G
EUR 84

Guanidine Hydrochloride

17353-25 500G
EUR 147

Guanidine Hydrochloride

18-206 500g/Unit
EUR 191.53
Description: Molecular/Proteomic Grade

Guanidine Hydrochloride

40700060-1 100 g
EUR 36.33

Guanidine Hydrochloride

40700060-2 500 g
EUR 89.21

Guanidine Hydrochloride

40700060-3 1 kg
EUR 153.37

Guanidine Hydrochloride

A2719-100G 100G
EUR 30.8
Description: Biotechnology

Guanidine Hydrochloride

A2719-500G 500G
EUR 97.9
Description: Biotechnology

Guanidine (hydrochloride)

HY-B0178A 50g
EUR 159.6

Guanidine Hydrochloride

G821245 10g
EUR 63
Description: 50-01-1

Guanidine hydrochloride

GB0242 100g
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Guanidine hydrochloride

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GE1914-1 1
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Guanidine hydrochloride, 99%

GE1914-100 100
EUR 15.9

Guanidine hydrochloride, 99%

GE1914-100G 100 g
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Guanidine hydrochloride, 99%

GE1914-1KG 1 kg
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Guanidine hydrochloride, 99%

GE1914-25 25
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Guanidine hydrochloride, 99%

GE1914-250 250
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Guanidine hydrochloride, 99%

GE1914-250G 250 g
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Guanidine hydrochloride, 99%

GE1914-25G 25 g
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Guanidine hydrochloride, 99%

GE1914-500 500
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Guanidine hydrochloride, 99%

GE1914-500G 500 g
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Guanidine hydrochloride powder

G05320 100G
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Guanidine-15N3 Hydrochloride

G821249 25mg
EUR 1200
Description: 121616-39-5

Guanidine hydrochloride solution, 8M

GE1001-100 100
EUR 120.5

Guanidine hydrochloride solution, 8M

GE1001-500 500
EUR 421.8

Guanidine hydrochloride solution, 6M

GE4739-1 1
EUR 711.9

Guanidine hydrochloride solution, 6M

GE4739-100 100
EUR 118.7

Guanidine hydrochloride solution, 6M

GE4739-500 500
EUR 408.1

Guanidine hydrochloride, Hi-LR™

GRM1504-100G 1 unit
EUR 46.61
Description: Guanidine hydrochloride, Hi-LR™

Guanidine hydrochloride, Hi-LR™

GRM1504-25G 1 unit
EUR 13.32
Description: Guanidine hydrochloride, Hi-LR™

Guanidine hydrochloride (6 M) solution

B1013-1L each
EUR 301.2

Guanidine hydrochloride (6 M) solution

B1013-4L each
EUR 705.6

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1-(2,4-difluorophenyl)guanidine hydrochloride

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Description: 1-(2,4-difluorophenyl)guanidine hydrochloride

1-(2,4-difluorophenyl)guanidine hydrochloride

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Description: 1-(2,4-difluorophenyl)guanidine hydrochloride

1-(2,4-difluorophenyl)guanidine hydrochloride

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1-(2,4-difluorophenyl)guanidine hydrochloride

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Description: 1-(2,4-difluorophenyl)guanidine hydrochloride

N-(3,4-Dimethoxyphenyl)guanidine Hydrochloride

D470625 10g
EUR 800
Description: 855890-38-9

N-Cyano-N'-(6-guanidinohexyl) Guanidine Hydrochloride

C655610 250mg
EUR 400

N-​(4-​Methyl-​2-​thiazolyl)​-guanidine hydrochloride

M331435 100mg
EUR 58
Description: 100599-91-5

N-(6-Aminohexyl)-N'-(6-guanidinohexyl) Guanidine Hydrochloride

A544590 50mg
EUR 6000

Casein, for Molecular Biology

MB279-100G 1 unit
EUR 110.77
Description: Casein, for Molecular Biology

Casein, for Molecular Biology

MB279-500G 1 unit
EUR 387.65
Description: Casein, for Molecular Biology

1-(4-Chloromethyl-2-thiazoyl)guanidine Hydrochloride Salt

C369545 1g
EUR 146

Urea, suitable for molecular biology

GE1210-1 1
EUR 58

Urea, suitable for molecular biology

GE1210-1KG 1 kg
EUR 106.8

Urea, suitable for molecular biology

GE1210-500 500
EUR 33.1

Urea, suitable for molecular biology

GE1210-500G 500 g
EUR 76.8

2-Mercaptoethanol ?For Molecular Biology

MB041-100ML 1 unit
EUR 9.02
Description: 2-Mercaptoethanol ?For Molecular Biology

2-Mercaptoethanol ?For Molecular Biology

MB041-500ML 1 unit
EUR 26.47
Description: 2-Mercaptoethanol ?For Molecular Biology

Sodium chloride, suitable for molecular biology

GE0307-1 1
EUR 45.2

Sucrose, GlenBiol, suitable for molecular biology

GC3201-1KG 1 kg
EUR 90

Molecular Biology Grade Water for RT-PCR

ML065-1.5ML 1 unit
EUR 7.82
Description: Molecular Biology Grade Water for RT-PCR

Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve

GS8780-2500 2500
EUR 249.8

DTT (Molecular Biology Grade)

CE131 5 g
EUR 93.6

DTT (Molecular Biology Grade)

CE132 10 g
EUR 133.2

DTT (Molecular Biology Grade)

CE133 25 g
EUR 243.6

NAD (Molecular Biology Grade)

CE196 1 g
EUR 72

NAD (Molecular Biology Grade)

CE197 5 g
EUR 165.6

NBT (Molecular Biology Grade)

CE209 1 g
EUR 123.6

NBT (Molecular Biology Grade)

CE210 5 g
EUR 360

Sucrose, GlenBiol™, suitable for molecular biology

GC3201-1 1
EUR 45.1

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40470006-1 100 mL
EUR 88.18

DMSO, Molecular Biology Grade

40470006-2 250 mL
EUR 150.19

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40470006-3 500 mL
EUR 279.26

EGTA, Molecular Biology Grade

40500028-2 50 g
EUR 106.43

EGTA, Molecular Biology Grade

40500028-3 100 g
EUR 177.58

EGTA, Molecular Biology Grade

40500028-4 500 g
EUR 603.19

EGTA, Molecular Biology Grade

40500028-5 1 kg
EUR 912.98

EGTA, Molecular Biology Grade

40500028-6 2 kg
EUR 1687.94

BCIP (Molecular Biology Grade)

CE108 250 mg
EUR 75.6

BCIP (Molecular Biology Grade)

CE109 1 g
EUR 108

DAPI (Molecular Biology Grade)

CE117 5 mg
EUR 72

DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 159.6

DAPI (Molecular Biology Grade)

CE119 100 mg
EUR 382.8

Tris (Molecular Biology Grade)

CE237 500 g
EUR 106.8

Tris (Molecular Biology Grade)

CE238 1 kg
EUR 153.6

Tris (Molecular Biology Grade)

CE239 5 kg
EUR 535.2

Pyridine, GlenBiol™, suitable for molecular biology

GS6659-2500 2500
EUR 240.3

Pyridine, GlenBiol™, suitable for molecular biology

GS6659-500 500
EUR 95.8

Formamide, GlenBiol™, suitable for molecular biology

GS9663-100 100
EUR 48.9

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CE114 1 g
EUR 66

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CE115 5 g
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CHAPS (Molecular Biology Grade)

CE116 25 g
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CE171 100 g
EUR 98.4

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CE172 500 g
EUR 268.8

HEPES (Molecular Biology Grade)

CE173 1 kg
EUR 424.8

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CE243 500 ml
EUR 62.4

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CE244 1 l
EUR 67.2

Molecular Biology Grade Water

ML024-100ML 1 unit
EUR 3.54
Description: Molecular Biology Grade Water

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ML024-10X100ML 1 unit
EUR 29.52
Description: Molecular Biology Grade Water

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ML024-10X500ML 1 unit
EUR 87.91
Description: Molecular Biology Grade Water

Molecular Biology Grade Water

ML024-500ML 1 unit
EUR 12.56
Description: Molecular Biology Grade Water

Molecular Biology Grade Water

ML064-100ML 1 unit
EUR 3.67
Description: Molecular Biology Grade Water

Molecular Biology Grade Water

ML064-10X100ML 1 unit
EUR 25.38
Description: Molecular Biology Grade Water

Molecular Biology Grade Water

ML064-500ML 1 unit
EUR 10.81
Description: Molecular Biology Grade Water

La curcumine régule la progression du cancer: focus sur les ARNnc et les voies de signalisation moléculaire 

Curcumin [(1E,6E) ‑1,7‑bis(4‑hydroxy‑3‑methoxyphenyl) hepta‑1,6‑diene‑3,5‑ dione] is a natural polyphenol derived from the rhizome of the turmeric plant Curcuma longa. Accumulated evidences have presented curcumin’s function in terms of anti-inflammatory, antioxidant properties, and especially anti-tumor activities. Studies demonstrated that curcumin could exert anti-tumor activity via multiple biological signaling pathways, such as PI3K/Akt, JAK/STAT, MAPK, Wnt/β-catenin, p53, NF-ĸB and apoptosis related signaling pathways. Moreover, Curcumin can inhibit tumor proliferation, angiogenesis, epithelial-mesenchymal transition (EMT), invasion and metastasis by regulating tumor related non-coding RNA (ncRNA) expression.

In this review, we summarized the roles of curcumin in regulating signaling pathways and ncRNAs in different kinds of cancers. We also discussed the regulatory effect of curcumin through inhibiting carcinogenic miRNA and up regulating tumor suppressive miRNA. Furthermore, we aim to illustrate the cross regulatory relationship between ncRNA and signaling pathways, further to get a better understanding of the anti-tumor mechanism of curcumin, thus lay a theoretical foundation for the clinical application of curcumin in the future.

Mécanismes moléculaires sous-jacents à l’activité antitumorale de la protéine de lactosérum de chameau contre les cellules de myélome multiple 

Treating drug-resistant cancer cells is a clinical challenge and it is also vital to screen for new cancer drugs. Multiple myeloma (MM) is a plasma cell clonal cancer that, despite many experimental therapeutics, remains incurable. In this study, two MM cell line lines U266 and RPMI 8226 were used to determine the impact of camel whey protein (CWP). The CWP IC50 was calculated by MTT examination, while the flow cytometry analysis was used to investigate the chemotaxis responses of MM cells in relation to CXCL12 and the pro-apoptotic effect of CHP. MM cells were treated with CWP and Western blot analysis was used to determine the underlying molecular mechanisms.

Dose and time based on the impact of CWP on the cell viability of MM cells with IC50 of 50 μg/ml, without affecting the viability of normal healthy PBMCs. CWP reduced chemotaxis of MM cells significantly from the CXC chemokine ligand 12 (CXCL12). Using Western blot analysis, we found that CWP decreased the activation of AKT, mTOR, PLCβ3, NFαB and ERK, which was mechanistically mediated by CXCL12/CXCR4. In both U266 and RPMI 8226, CWP induced apoptosis by upregulating cytochrome C expression.

In addition, CWP mediated the growth arrest of MM cells by robustly decreasing the expression of the anti-apoptotic Bcl-2 family members Bcl-2, Bcl-XL and Mcl-1. Conversely, the expression of pro-apoptotic Bcl-2 family members Bak, Bax and Bim was increased after treatment with CWP. Our data indicates CWP’s therapeutic potential for MM cells.

Identification moléculaire de Campanulotes bidentatus Scopoli, 1763 (Phthiraptera, Philopteridae) infectant le pigeon domestique Columba livia d’Arabie saoudite. 

The taxonomy of the order Phthiraptera is unstable and still problematic to researchers. Most of the current taxon classifications are mainly based on morphological features. <i>Campanulotes bidentatus</i> belongs to the chewing lice of the Philopteridae family that mostly parasitic on birds. There is a lack of sequence data and phylogenetic analyses on the family Philopteridae. In the current study, <i>C. bidentatus</i> was collected from the domestic pigeon <i>Columba livia</i> and identified morphologically and molecularly based on the mitochondrial cytochrome <i>c</i> oxidase subunit 1 gene (<i>COI</i>).

The infection rate of the <i>Campanulotes</i> genus was approximately 58.82% in this study. Phylogenetic analysis based on the mt <i>COI</i> gene was informative for members of Philopteridae and the group taxon genera formed distinct clades. Future studies were recommended using the <i>16s rRNA</i> to enhance the tree topology and obtain clear differentiation between genera.

Escherichia coli multirésistante dans le lait cru: caractérisation moléculaire et impact potentiel de l’urine de chameau en tant qu’agent antibactérien 

Raw milk is one of the most important vehicles for transmitting various pathogens, especially Escherichia coli (E. coli). Multidrug-resistant pathogens are highly prevalent among mastitic cows in various dairy farms worldwide. Therefore, our current study is based on the identification of E. coli from mastitic cow’s milk and their resistance to various antibacterial agents. As well, the impact of camel’s urine on multi-drug resistant E. coli were also evaluated. Thirty-three E. coli isolates were recovered from 254 milk samples. All strains were initially identified phenotypically by culturing on specific media and Vitek 2 Compact System. The protein fingerprinting technique was used as a confirmatory method. The Stx1Stx2 and eae genes were also verified by polymerase chain reaction (PCR). The antimicrobial resistance of E. coli strains was tested by the Vitek 2 AST-GN69 cards.

Thirty multi-drug resistant E. coli strains (20 from mastitic milk and 10 from clinical samples) were laboratory tested with different concentrations (100%, 75%, 50% and 25%) of virgin and breeding camel’s urine, using the paper disc diffusion method. Our findings showed that 93.94% of E. coli strains were recognized by the Vitek™ 2 system. The results of proteomic investigation illustrated that 100% of E. coli strains were identified at log values ≥2.00. The genotypic identification of the three virulence genes illustrated that 90.1%, 63.64%, and 30.55% of E. coli strains were able to carry the Stx1eae, and Stx2 genes, respectively.

Most strains of E. coli showed strong resistance against cefazolin (78.79%), ceftazidime (66.67%), cefotaxime (60.61%), ceftriaxone (54.55%), and cefepime (39.40%). The results of the antibacterial effect of camel’s urine revealed that the mean inhibitory zones of virgin camel’s urine were 28 mm, 17 mm, and 14 mm, for the concentrations of 100%, 75%, and 50%, respectively. Whereas; the inhibitory zones for the breeding camel’s urine were 18 mm, 0 mm, and 0 mm, for the concentrations of 100%, 75%, and 50%, respectively. We concluded that the majority of E. coli strains were able to harbor some virulence genes and resist many antibiotics. Our study also provided a robust evidence that the camel’s urine, particularly from the virgin camels has robust antimicrobial activity against multidrug-resistant E. coli strains.

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