Système immunitaire et méthamphétamine

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Système immunitaire et méthamphétamine: base moléculaire d’une relation 

Use of methamphetamine (Meth) as a drug of abuse is on the rise worldwide. Besides its effect on the function of the brain, Meth has detrimental effects on how the immune system functions. As documented in the literature, various experimental models (cellular, animal, mice, and non-human primates) have been used that have contributed to the overall knowledge about immune system impairments from Meth exposure. It has to be noted that while Meth is used in very few treatments, it affects a broad range of biological mechanisms, not only immune regulation, in a negative manner.

Undoubtfully, the effect of Meth is highly complex; moreover, the initial molecular triggers remain unknown. Analyses of available literature suggests that the effect of Meth is not prompted by one underlying mechanism. Whether the effect of Meth is acute or long-lasting, the overall effect is negative. Further advancement of our knowledge on Meth’s specific actions will require systematic experimental approaches using all available models. In addition, bioinformatic analyses are necessary to build a comprehensive model as a needed tool to fill the gap in knowledge.

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Guanidine Thiocyanate (Molecular Biology Grade)

CE166 1 kg
EUR 307.2

Guanidine hydrochloride

GB0242 100g
EUR 80.88

Guanidine (hydrochloride)

HY-B0178A 50g
EUR 159.6

Guanidine hydrochloride, 99%

GE1914-100G 100 g
EUR 55.2

Guanidine hydrochloride, 99%

GE1914-1KG 1 kg
EUR 132

Guanidine hydrochloride, 99%

GE1914-250G 250 g
EUR 64.8

Guanidine hydrochloride, 99%

GE1914-25G 25 g
EUR 50.4

Guanidine hydrochloride, 99%

GE1914-500G 500 g
EUR 88.8

Guanidine hydrochloride, 99%

GE1914-1 1
EUR 79.1

Guanidine hydrochloride, 99%

GE1914-100 100
EUR 15.9

Guanidine hydrochloride, 99%

GE1914-25 25
EUR 13

Guanidine hydrochloride, 99%

GE1914-250 250
EUR 23.8

Guanidine hydrochloride, 99%

GE1914-500 500
EUR 43.5

Guanidine hydrochloride solution, 8M

GE1001-100 100
EUR 120.5

Guanidine hydrochloride solution, 8M

GE1001-500 500
EUR 421.8

Guanidine hydrochloride solution, 6M

GE4739-1 1
EUR 711.9

Guanidine hydrochloride solution, 6M

GE4739-100 100
EUR 118.7

Guanidine hydrochloride solution, 6M

GE4739-500 500
EUR 408.1

Guanidine hydrochloride (6 M) solution

B1013-1L each
EUR 301.2

Guanidine hydrochloride (6 M) solution

B1013-4L each
EUR 705.6

Guanidine Thiocyanate Molecular/Proteomic Grade

18-207 500g/Unit
EUR 479

Urea, suitable for molecular biology

GE1210-1KG 1 kg
EUR 106.8

Urea, suitable for molecular biology

GE1210-500G 500 g
EUR 76.8

Urea, suitable for molecular biology

GE1210-1 1
EUR 58

Urea, suitable for molecular biology

GE1210-500 500
EUR 33.1

Sodium chloride, suitable for molecular biology

GE0307-1 1
EUR 45.2

Sucrose, GlenBiol, suitable for molecular biology

GC3201-1KG 1 kg
EUR 90

DTT (Molecular Biology Grade)

CE131 5 g
EUR 93.6

DTT (Molecular Biology Grade)

CE132 10 g
EUR 133.2

DTT (Molecular Biology Grade)

CE133 25 g
EUR 243.6

NAD (Molecular Biology Grade)

CE196 1 g
EUR 72

NAD (Molecular Biology Grade)

CE197 5 g
EUR 165.6

NBT (Molecular Biology Grade)

CE209 1 g
EUR 123.6

NBT (Molecular Biology Grade)

CE210 5 g
EUR 360

BCIP (Molecular Biology Grade)

CE108 250 mg
EUR 75.6

BCIP (Molecular Biology Grade)

CE109 1 g
EUR 108

DAPI (Molecular Biology Grade)

CE117 5 mg
EUR 72

DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 159.6

DAPI (Molecular Biology Grade)

CE119 100 mg
EUR 382.8

Tris (Molecular Biology Grade)

CE237 500 g
EUR 106.8

Tris (Molecular Biology Grade)

CE238 1 kg
EUR 153.6

Tris (Molecular Biology Grade)

CE239 5 kg
EUR 535.2

100mL Molecular Biology Grade

46-000-CI PK6
EUR 74.4

500mL Molecular Biology Grade

46-000-CV PK6
EUR 138

CHAPS (Molecular Biology Grade)

CE114 1 g
EUR 66

CHAPS (Molecular Biology Grade)

CE115 5 g
EUR 157.2

CHAPS (Molecular Biology Grade)

CE116 25 g
EUR 492

HEPES (Molecular Biology Grade)

CE171 100 g
EUR 98.4

HEPES (Molecular Biology Grade)

CE172 500 g
EUR 268.8

HEPES (Molecular Biology Grade)

CE173 1 kg
EUR 424.8

Water (Molecular Biology Grade)

CE243 500 ml
EUR 62.4

Water (Molecular Biology Grade)

CE244 1 l
EUR 67.2

Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve

GS8780-2500 2500
EUR 249.8

Glycine (Molecular Biology Grade)

CE158 1 kg
EUR 84

Glycine (Molecular Biology Grade)

CE159 5 kg
EUR 228

Tween20 (Molecular Biology Grade)

CE242 1 l
EUR 106.8

Sucrose, GlenBiol™, suitable for molecular biology

GC3201-1 1
EUR 45.1

Lysozyme (Molecular Biology Grade)

CE188 1 g
EUR 70.8

Lysozyme (Molecular Biology Grade)

CE189 10 g
EUR 247.2

Pyridine, GlenBiol™, suitable for molecular biology

GS6659-2500 2500
EUR 240.3

Pyridine, GlenBiol™, suitable for molecular biology

GS6659-500 500
EUR 95.8

Formamide, GlenBiol™, suitable for molecular biology

GS9663-100 100
EUR 48.9

1L Molecular Biology Grade Water

46-000-CM PK6
EUR 196.8

Tween 20, Molecular Biology Grade

T9100-010 100ml
EUR 86.4

Tween 20, Molecular Biology Grade

T9100-050 500ml
EUR 133.2

Tween 20, Molecular Biology Grade

T9100-100 1L
EUR 160.8

D(+)-Sucrose (Molecular Biology Grade)

CE224 500 g
EUR 67.2

D(+)-Sucrose (Molecular Biology Grade)

CE225 1 kg
EUR 84

D(+)-Sucrose (Molecular Biology Grade)

CE226 5 kg
EUR 207.6

Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve

GS3406-2500 2500
EUR 116.2

Glycerol 87 % (Molecular Biology Grade)

CE154 1 l
EUR 93.6

MOPS buffer (Molecular Biology Grade)

CE194 100 g
EUR 102

MOPS buffer (Molecular Biology Grade)

CE195 250 g
EUR 169.2

Agarose, low EEO, GlenBiol, suitable for molecular biology

GE6258-100G 100 g
EUR 217.2

Formamide deionized (Molecular Biology Grade)

CE145 500 ml
EUR 87.6

Formamide deionized (Molecular Biology Grade)

CE146 1 l
EUR 120

TritonX-100 (Molecular Biology Grade)

CE240 500 ml
EUR 67.2

TritonX-100 (Molecular Biology Grade)

CE241 1 l
EUR 79.2

Dimethylsulfoxide (Molecular Biology Grade)

CE120 100 ml
EUR 66

Dimethylsulfoxide (Molecular Biology Grade)

CE121 500 ml
EUR 110.4

Dimethylformamide, GlenBiol™, suitable for molecular biology

GS6580-2500 2500
EUR 107.3

Water, distilled, GlenBiol™, suitable for molecular biology

GK8512-1L 1 l
EUR 92.4

Water, distilled, GlenBiol™, suitable for molecular biology

GK8512-1 1
EUR 60.1

Acetonitrile 50, GlenBiol™, suitable for molecular biology

GS0247-1 1
EUR 40.7

Acetonitrile 50, GlenBiol™, suitable for molecular biology

GS0247-2500 2500
EUR 61.3

Acetonitrile 10, GlenBiol™, suitable for molecular biology

GS0969-1 1
EUR 47

Acetonitrile 10, GlenBiol™, suitable for molecular biology

GS0969-2500 2500
EUR 73.9

Acetonitrile 30, GlenBiol™, suitable for molecular biology

GS8649-1 1
EUR 43.1

Acetonitrile 30, GlenBiol™, suitable for molecular biology

GS8649-2500 2500
EUR 66.5

Water, Ultrapure Molecular Biology Grade

41024-4L 4L
EUR 145.2
Description: Minimum order quantity: 1 unit of 4L

Bis-Acrylamid (Molecular Biology Grade)

CE110 50 g
EUR 94.8

Bis-Acrylamid (Molecular Biology Grade)

CE111 250 g
EUR 259.2

Sodium chloride (Molecular Biology Grade)

CE205 500 g
EUR 62.4

Sodium chloride (Molecular Biology Grade)

CE206 1 kg
EUR 70.8

Sodium chloride (Molecular Biology Grade)

CE207 5 kg
EUR 123.6

Phenol, (Carbolic acid) Double distilled for Molecular Biology

PD0252 500g
EUR 192.59

Ammonia solution, GlenBiol™, suitable for molecular biology

GS8853-100 100
EUR 91.2

Ammonium sulfate (Molecular Biology Grade)

CE105 250 g
EUR 55.2

Ammonium sulfate (Molecular Biology Grade)

CE106 1 kg
EUR 72

Ammonium sulfate (Molecular Biology Grade)

CE107 5 kg
EUR 153.6

Urea Crystalline (Molecular Biology Grade)

CE167 1 kg
EUR 72

Urea Crystalline (Molecular Biology Grade)

CE168 5 kg
EUR 181.2

Agarose, low EEO, GlenBiol™, suitable for molecular biology

GE6258-100 100
EUR 150.4

Agarose, low EEO, GlenBiol™, suitable for molecular biology

GE6258-25 25
EUR 60.1

SSC Buffer (20X) (Molecular Biology Grade)

CE229 1 l
EUR 86.4

XTT sodium salt (Molecular Biology Grade)

CE250 100 mg
EUR 208.8

XTT sodium salt (Molecular Biology Grade)

CE251 500 mg
EUR 612

Glycerol waterfree (Molecular Biology Grade)

CE155 500 ml
EUR 78

Glycerol waterfree (Molecular Biology Grade)

CE156 1 l
EUR 102

Glycerol waterfree (Molecular Biology Grade)

CE157 2.5 l
EUR 170.4

La curcumine régule la progression du cancer: focus sur les ARNnc et les voies de signalisation moléculaire 

Curcumin [(1E,6E) ‑1,7‑bis(4‑hydroxy‑3‑methoxyphenyl) hepta‑1,6‑diene‑3,5‑ dione] is a natural polyphenol derived from the rhizome of the turmeric plant Curcuma longa. Accumulated evidences have presented curcumin’s function in terms of anti-inflammatory, antioxidant properties, and especially anti-tumor activities. Studies demonstrated that curcumin could exert anti-tumor activity via multiple biological signaling pathways, such as PI3K/Akt, JAK/STAT, MAPK, Wnt/β-catenin, p53, NF-ĸB and apoptosis related signaling pathways. Moreover, Curcumin can inhibit tumor proliferation, angiogenesis, epithelial-mesenchymal transition (EMT), invasion and metastasis by regulating tumor related non-coding RNA (ncRNA) expression.

In this review, we summarized the roles of curcumin in regulating signaling pathways and ncRNAs in different kinds of cancers. We also discussed the regulatory effect of curcumin through inhibiting carcinogenic miRNA and up regulating tumor suppressive miRNA. Furthermore, we aim to illustrate the cross regulatory relationship between ncRNA and signaling pathways, further to get a better understanding of the anti-tumor mechanism of curcumin, thus lay a theoretical foundation for the clinical application of curcumin in the future.

Mécanismes moléculaires sous-jacents à l’activité antitumorale de la protéine de lactosérum de chameau contre les cellules de myélome multiple 

Treating drug-resistant cancer cells is a clinical challenge and it is also vital to screen for new cancer drugs. Multiple myeloma (MM) is a plasma cell clonal cancer that, despite many experimental therapeutics, remains incurable. In this study, two MM cell line lines U266 and RPMI 8226 were used to determine the impact of camel whey protein (CWP). The CWP IC50 was calculated by MTT examination, while the flow cytometry analysis was used to investigate the chemotaxis responses of MM cells in relation to CXCL12 and the pro-apoptotic effect of CHP. MM cells were treated with CWP and Western blot analysis was used to determine the underlying molecular mechanisms.

Dose and time based on the impact of CWP on the cell viability of MM cells with IC50 of 50 μg/ml, without affecting the viability of normal healthy PBMCs. CWP reduced chemotaxis of MM cells significantly from the CXC chemokine ligand 12 (CXCL12). Using Western blot analysis, we found that CWP decreased the activation of AKT, mTOR, PLCβ3, NFαB and ERK, which was mechanistically mediated by CXCL12/CXCR4. In both U266 and RPMI 8226, CWP induced apoptosis by upregulating cytochrome C expression.

In addition, CWP mediated the growth arrest of MM cells by robustly decreasing the expression of the anti-apoptotic Bcl-2 family members Bcl-2, Bcl-XL and Mcl-1. Conversely, the expression of pro-apoptotic Bcl-2 family members Bak, Bax and Bim was increased after treatment with CWP. Our data indicates CWP’s therapeutic potential for MM cells.

Identification moléculaire de Campanulotes bidentatus Scopoli, 1763 (Phthiraptera, Philopteridae) infectant le pigeon domestique Columba livia d’Arabie saoudite. 

The taxonomy of the order Phthiraptera is unstable and still problematic to researchers. Most of the current taxon classifications are mainly based on morphological features. <i>Campanulotes bidentatus</i> belongs to the chewing lice of the Philopteridae family that mostly parasitic on birds. There is a lack of sequence data and phylogenetic analyses on the family Philopteridae. In the current study, <i>C. bidentatus</i> was collected from the domestic pigeon <i>Columba livia</i> and identified morphologically and molecularly based on the mitochondrial cytochrome <i>c</i> oxidase subunit 1 gene (<i>COI</i>).

The infection rate of the <i>Campanulotes</i> genus was approximately 58.82% in this study. Phylogenetic analysis based on the mt <i>COI</i> gene was informative for members of Philopteridae and the group taxon genera formed distinct clades. Future studies were recommended using the <i>16s rRNA</i> to enhance the tree topology and obtain clear differentiation between genera.

Escherichia coli multirésistante dans le lait cru: caractérisation moléculaire et impact potentiel de l’urine de chameau en tant qu’agent antibactérien 

Raw milk is one of the most important vehicles for transmitting various pathogens, especially Escherichia coli (E. coli). Multidrug-resistant pathogens are highly prevalent among mastitic cows in various dairy farms worldwide. Therefore, our current study is based on the identification of E. coli from mastitic cow’s milk and their resistance to various antibacterial agents. As well, the impact of camel’s urine on multi-drug resistant E. coli were also evaluated. Thirty-three E. coli isolates were recovered from 254 milk samples. All strains were initially identified phenotypically by culturing on specific media and Vitek 2 Compact System. The protein fingerprinting technique was used as a confirmatory method. The Stx1Stx2 and eae genes were also verified by polymerase chain reaction (PCR). The antimicrobial resistance of E. coli strains was tested by the Vitek 2 AST-GN69 cards.

Thirty multi-drug resistant E. coli strains (20 from mastitic milk and 10 from clinical samples) were laboratory tested with different concentrations (100%, 75%, 50% and 25%) of virgin and breeding camel’s urine, using the paper disc diffusion method. Our findings showed that 93.94% of E. coli strains were recognized by the Vitek™ 2 system. The results of proteomic investigation illustrated that 100% of E. coli strains were identified at log values ≥2.00. The genotypic identification of the three virulence genes illustrated that 90.1%, 63.64%, and 30.55% of E. coli strains were able to carry the Stx1eae, and Stx2 genes, respectively.

Most strains of E. coli showed strong resistance against cefazolin (78.79%), ceftazidime (66.67%), cefotaxime (60.61%), ceftriaxone (54.55%), and cefepime (39.40%). The results of the antibacterial effect of camel’s urine revealed that the mean inhibitory zones of virgin camel’s urine were 28 mm, 17 mm, and 14 mm, for the concentrations of 100%, 75%, and 50%, respectively. Whereas; the inhibitory zones for the breeding camel’s urine were 18 mm, 0 mm, and 0 mm, for the concentrations of 100%, 75%, and 50%, respectively. We concluded that the majority of E. coli strains were able to harbor some virulence genes and resist many antibiotics. Our study also provided a robust evidence that the camel’s urine, particularly from the virgin camels has robust antimicrobial activity against multidrug-resistant E. coli strains.

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