Thrombin Plasma prep for Exosome
Efficient isolation of exosomes from plasma
“Therefore, we looked to the ExoQuick method for further studies, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models.” By isolating exosomes from plasma, fibrinogen and fibrin can prevent effective recovery. By pretreating plasma with thrombin, fibrinogen can be converted to fibrin and easily pre-cleared by a simple centrifugation step. The resulting serum-like solution can now be treated with ExoQuick, the original formulation, for quantitative isolation of exosomes that are compatible with high-throughput methods.
ExoQuick’s fast, non-ultracentrifugation method:
- Save time and labor
- It is easily scalable
- Keep a precious sample
- It offers high yields of high-quality and functional exosomes.
- It can be used to isolate exosomes for a wide range of downstream applications, including
- Biomarker studies
- Exosomal miRNA profiles
- Exosomal proteomics
- Lipidomics / exosomal metabolomics
- Functional studies, such as cell-to-cell signaling.
- Basic biology, such as its role in tumorigenesis.
ExoQuick is a proprietary polymer that gently precipitates exosomes. For the isolation of exosomes from plasma, you can first defibrinate your sample with thrombin and then continue with the standard ExoQuick protocol of pre-cleaning your sample of cells and cell debris, adding the appropriate amount of ExoQuick, refrigerating, and then centrifuging (see product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in a suitable solution.
In electron microscopy studies, exosomes isolated with ExoQuick appear similar to exosomes isolated by ultracentrifugation1-2, and these exosomes are also active in numerous functional assays1-3.
Exosomes isolated with ExoQuick can be used for all types of protein profiling and characterization studies, such as mass spectrometry, Western blotting, ELISA, and more. Higher protein yields are obtained by ExoQuick purification than by chromatography, DynaBeads, or ultracentrifugation.
Exosomes isolated with ExoQuick also provide excellent samples for studying exosome-associated nucleic acids, such as microRNA, siRNA, and even mRNA. Quantitative analytical techniques such as qPCR, microarray studies, and next-generation sequencing are compatible with nucleic acids isolated from exosomes purified with ExoQuick.
Backed by a growing number of publications, ExoQuick is often the best choice for researchers working with low sample volumes, such as clinical research samples or small animal models.