Quality level: 300
Biological source: Streptomyces alboniger
Sterility: 0.2 μm filtered
Test: > 98% (HPLC)
Concentration: 10 mg / mL in H2O
cell culture | mammal: suitable
H2O: soluble 10 mg / mL
protein synthesis | interferes
The spectrum of antibiotic activity
- Gram-positive bacteria
Related categories: Antibiotics
Chemical structure: peptidyl nucleoside
Puromycin is an aminonucleoside antibiotic derived from Streptomyces alboniger. It allows the selection of cells that contain the resistance gene puromycin N-acetyltransferase (PAC). It has been used to study the viability of vascular smooth muscle cells after treatment in the rabbit model. Puromycin is used to produce transgenic piglets with an enhanced green fluorescent protein (EGFP) after somatic cell cloning and embryo transfer.
10 mL in glass bottle
Biochemical / Physiological Actions
Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination that acts as an analog of the 3 ‘end of aminoacyl-tRNA. Puromycin also acts as a reversible inhibitor of dipeptidyl peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.
Resistance mode: Puromycin acetyltransferase is an effective resistance gene.
Antimicrobial spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli, and weaker against gram-negative microorganisms. Puromycin can prevent the growth of bacteria, protozoa, algae, and mammalian cells and works quickly, killing 99% of cells within 2 days.
This product is concentrated to 10 mg / mL in water and filtered 0.2 µm. It has been tested in HeLa cells for cell growth arrest and cell selection after transfection of the pac resistance gene.
Keep the container tightly closed in a dry and well-ventilated place.