Puromycin solution

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Quality level: 300

Biological source: Streptomyces alboniger

Sterility: 0.2 μm filtered

Test: > 98% (HPLC)

Form: solution

Concentration: 10 mg / mL in H2O

Application(s)

cell culture | mammal: suitable

Solubility

H2O: soluble 10 mg / mL

Action mode

protein synthesis | interferes

The spectrum of antibiotic activity

  • Gram-positive bacteria
  • neoplastic
  • parasites

Related categories: Antibiotics

General description

Chemical structure: peptidyl nucleoside

Request

Puromycin is an aminonucleoside antibiotic derived from Streptomyces alboniger. It allows the selection of cells that contain the resistance gene puromycin N-acetyltransferase (PAC). It has been used to study the viability of vascular smooth muscle cells after treatment in the rabbit model. Puromycin is used to produce transgenic piglets with an enhanced green fluorescent protein (EGFP) after somatic cell cloning and embryo transfer.

Packaging

10 mL in glass bottle

Biochemical / Physiological Actions

Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination that acts as an analog of the 3 ‘end of aminoacyl-tRNA. Puromycin also acts as a reversible inhibitor of dipeptidyl peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.

Resistance mode: Puromycin acetyltransferase is an effective resistance gene.

Antimicrobial spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli, and weaker against gram-negative microorganisms. Puromycin can prevent the growth of bacteria, protozoa, algae, and mammalian cells and works quickly, killing 99% of cells within 2 days.

Preparation note

This product is concentrated to 10 mg / mL in water and filtered 0.2 µm. It has been tested in HeLa cells for cell growth arrest and cell selection after transfection of the pac resistance gene.

Other notes

Keep the container tightly closed in a dry and well-ventilated place.

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