Détection moléculaire de Tropheryma whipplei

0 Comments
pcipresse

Détection moléculaire de Tropheryma whipplei , Cryptosporidium spp. Et Giardia lamblia parmi les échantillons de maladie cœliaque 

Background: Celiac illness (CD) is without doubt one of the most typical issues, ensuing from each environmental (gluten) and genetic elements. The scientific options of the Iranian CD are nonetheless unknown and there’s inadequate details about the atypical presentation of CD from Iran. As, many earlier reviews revealed an affiliation between managed protozoal infections and the CD in keeping with cytokines manufacturing, the intention of this research was to find out the prevalence of CD and doable co-infection with probably the most prevalent protozoal infections together with Tropheryma whippleiCryptosporidium, and Giardia duodenalis amongst CD samples.

Supplies and strategies: On this research, from April 2014 to November 2016, 524 samples had been obtained from small gut of sufferers with gastrointestinal illnesses referring to the Pathology Division of Namazi Hospital, Shiraz, Iran. Multiplex polymerase chain response assay was then carried out on the histological optimistic CD samples for the prevalence of the microorganisms.

Outcomes: Sixty-four (12.21%) sufferers had been identified as having CD by histopathological examination. The prevalence of T. whipplei and Cryptosporidium spp. was 19 (29.69%) and eight (12.5%) respectively, amongst CD optimistic samples there was no optimistic pattern for Giardia lamblia.

Conclusion: The prevalence of CD among the many southwestern Iranian inhabitants was excessive and comparable with different areas of Iran in addition to many different international locations. Moreover, no important affiliation between the presence of T. whippleiCryptosporidium spp., and degree of the histopathological modifications of villi within the CD was noticed (P > 0.05).

pcipresse
pcipresse

CHAPS (Molecular Biology Grade)

CE115 5 g
EUR 157.2

CHAPS (Molecular Biology Grade)

CE116 25 g
EUR 492

DAPI (Molecular Biology Grade)

CE117 5 mg
EUR 72

DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 159.6

DAPI (Molecular Biology Grade)

CE119 100 mg
EUR 382.8

Dimethylsulfoxide (Molecular Biology Grade)

CE120 100 ml
EUR 66

Dimethylsulfoxide (Molecular Biology Grade)

CE121 500 ml
EUR 110.4

DTT (Molecular Biology Grade)

CE131 5 g
EUR 93.6

DTT (Molecular Biology Grade)

CE132 10 g
EUR 133.2

DTT (Molecular Biology Grade)

CE133 25 g
EUR 243.6

Glycine (Molecular Biology Grade)

CE158 1 kg
EUR 84

Glycine (Molecular Biology Grade)

CE159 5 kg
EUR 228

HEPES (Molecular Biology Grade)

CE171 100 g
EUR 98.4

HEPES (Molecular Biology Grade)

CE172 500 g
EUR 268.8

HEPES (Molecular Biology Grade)

CE173 1 kg
EUR 424.8

Lysozyme (Molecular Biology Grade)

CE188 1 g
EUR 70.8

Lysozyme (Molecular Biology Grade)

CE189 10 g
EUR 247.2

NAD (Molecular Biology Grade)

CE196 1 g
EUR 72

NAD (Molecular Biology Grade)

CE197 5 g
EUR 165.6

NBT (Molecular Biology Grade)

CE209 1 g
EUR 123.6

NBT (Molecular Biology Grade)

CE210 5 g
EUR 360

Tris (Molecular Biology Grade)

CE237 500 g
EUR 106.8

Tris (Molecular Biology Grade)

CE238 1 kg
EUR 153.6

Tris (Molecular Biology Grade)

CE239 5 kg
EUR 535.2

Tween20 (Molecular Biology Grade)

CE242 1 l
EUR 106.8

Water (Molecular Biology Grade)

CE243 500 ml
EUR 62.4

Water (Molecular Biology Grade)

CE244 1 l
EUR 67.2

100mL Molecular Biology Grade

46-000-CI PK6
EUR 70.68

500mL Molecular Biology Grade

46-000-CV PK6
EUR 124.26

Water, Ultrapure Molecular Biology Grade

41024-4L 4L
EUR 145.2
Description: Minimum order quantity: 1 unit of 4L

Ammonium sulfate (Molecular Biology Grade)

CE105 250 g
EUR 55.2

Ammonium sulfate (Molecular Biology Grade)

CE106 1 kg
EUR 72

Ammonium sulfate (Molecular Biology Grade)

CE107 5 kg
EUR 153.6

Bis-Acrylamid (Molecular Biology Grade)

CE110 50 g
EUR 94.8

Bis-Acrylamid (Molecular Biology Grade)

CE111 250 g
EUR 259.2

Formamide deionized (Molecular Biology Grade)

CE145 500 ml
EUR 87.6

Formamide deionized (Molecular Biology Grade)

CE146 1 l
EUR 120

Glycerol 87 % (Molecular Biology Grade)

CE154 1 l
EUR 93.6

Glycerol waterfree (Molecular Biology Grade)

CE155 500 ml
EUR 78

Glycerol waterfree (Molecular Biology Grade)

CE156 1 l
EUR 102

Glycerol waterfree (Molecular Biology Grade)

CE157 2.5 l
EUR 170.4

Guanidine - Hydrochloride (Molecular Biology Grade)

CE160 100 g
EUR 93.6

Guanidine - Hydrochloride (Molecular Biology Grade)

CE161 250 g
EUR 153.6

Guanidine - Hydrochloride (Molecular Biology Grade)

CE162 500 g
EUR 232.8

Guanidine - Hydrochloride (Molecular Biology Grade)

CE163 1 kg
EUR 352.8

Guanidine Thiocyanate (Molecular Biology Grade)

CE164 100 g
EUR 86.4

Guanidine Thiocyanate (Molecular Biology Grade)

CE165 500 g
EUR 192

Guanidine Thiocyanate (Molecular Biology Grade)

CE166 1 kg
EUR 307.2

MOPS buffer (Molecular Biology Grade)

CE194 100 g
EUR 102

MOPS buffer (Molecular Biology Grade)

CE195 250 g
EUR 169.2

Sodium chloride (Molecular Biology Grade)

CE205 500 g
EUR 62.4

Sodium chloride (Molecular Biology Grade)

CE206 1 kg
EUR 70.8

Sodium chloride (Molecular Biology Grade)

CE207 5 kg
EUR 123.6

D(+)-Sucrose (Molecular Biology Grade)

CE224 500 g
EUR 67.2

D(+)-Sucrose (Molecular Biology Grade)

CE225 1 kg
EUR 84

D(+)-Sucrose (Molecular Biology Grade)

CE226 5 kg
EUR 207.6

Tris - Hydrochloride (Molecular Biology Grade)

CE234 250 g
EUR 99.6

Tris - Hydrochloride (Molecular Biology Grade)

CE235 500 g
EUR 144

Tris - Hydrochloride (Molecular Biology Grade)

CE236 1 kg
EUR 223.2

TritonX-100 (Molecular Biology Grade)

CE240 500 ml
EUR 67.2

TritonX-100 (Molecular Biology Grade)

CE241 1 l
EUR 79.2

Tween 20, Molecular Biology Grade

T9100-010 100ml
EUR 86.4

Tween 20, Molecular Biology Grade

T9100-050 500ml
EUR 133.2

Tween 20, Molecular Biology Grade

T9100-100 1L
EUR 160.8

1L Molecular Biology Grade Water

46-000-CM PK6
EUR 177.84

Water, distilled, GlenBiol™, suitable for molecular biology

GK8512-1L 1 l
EUR 92.4

Agarose, low EEO, GlenBiol, suitable for molecular biology

GE6258-100G 100 g
EUR 217.2

Crystalline

5-01010 10mg Ask for price

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE135 250 g
EUR 72

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE136 500 g
EUR 86.4

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE137 1 kg
EUR 124.8

EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade)

CE138 5 kg
EUR 418.8

D(+)-Glucose waterfree (Molecular Biology Grade)

CE148 500 g
EUR 67.2

D(+)-Glucose waterfree (Molecular Biology Grade)

CE149 1 kg
EUR 75.6

D(+)-Glucose waterfree (Molecular Biology Grade)

CE150 5 kg
EUR 180

Yeast extract powder (Molecular Biology Grade)

CE169 500 g
EUR 133.2

Hyaluronidase Grade I (Molecular Biology Grade)

CE174 1 g
EUR 232.8

Hyaluronidase Grade I (Molecular Biology Grade)

CE175 5 g
EUR 920.4

Magnesium acetate - Tetrahydrate (Molecular Biology Grade)

CE190 500 g
EUR 98.4

NADH - Disodium salt (Molecular Biology Grade)

CE198 1 g
EUR 91.2

NADH - Disodium salt (Molecular Biology Grade)

CE199 5 g
EUR 244.8

NADP - sodium salt (Molecular Biology Grade)

CE200 250 mg
EUR 92.4

NADP - sodium salt (Molecular Biology Grade)

CE201 1 g
EUR 190.8

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE202 25 mg
EUR 70.8

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE203 100 mg
EUR 126

NADPH - Tetrasodium salt (Molecular Biology Grade)

CE204 500 mg
EUR 374.4

SSC Buffer (20X) (Molecular Biology Grade)

CE229 1 l
EUR 86.4

XTT sodium salt (Molecular Biology Grade)

CE250 100 mg
EUR 208.8

XTT sodium salt (Molecular Biology Grade)

CE251 500 mg
EUR 612

Albumin fraction V (pH7,0) (Molecular Biology Grade)

CE100 50 g
EUR 128.4

Sensibilité du take a look at moléculaire dans la salive pour la détection du COVID-19 chez les sufferers pédiatriques atteints d’affections concomitantes

Background: The reference customary for the molecular diagnostic testing for COVID-19 is the usage of nasopharyngeal or mixed nasopharyngeal and oropharyngeal (NP/OP) swabs. Saliva has been proposed as a minimally invasive specimen whose assortment reduces the dangers for well being care personnel.

Goal: To evaluate the suitability of saliva for COVID-19 analysis as a substitute of the reference customary NP/OP swab within the setting of a tertiary care pediatric unit.

Examine design: A paired research based mostly in the possible cohort design in sufferers suspected of getting COVID-19.

Strategies: RT-PCR was used to detect SARS-CoV-2 in paired samples of saliva and NP/OP swab collected from Might by August 2020 from 156 pediatric contributors, of whom 128 has a minimum of one comorbidity and 91 confirmed scientific signs associated to SARS-CoV-2 an infection. Moreover, we studied a bunch of 326 members of the hospital workers, of whom 271 had signs associated to SARS-CoV-2 an infection.

Outcomes: Within the group of pediatric contributors the sensitivity of the diagnostic take a look at in saliva was 82.3% (95% CI 56.6-96.2) and the specificity 95.6% (95% CI 90.8-98.4). The prevalence of COVID-19 was 10.9% (17/156). In 6 of the 23 contributors who examined optimistic for SARS-CoV-2 in a minimum of one specimen sort, the virus was detected in saliva however not in NP/OP swab, whereas in Three contributors the NP/OP swab was optimistic and saliva unfavourable. Within the group of adults, the sensitivity of the take a look at in saliva was 77.8% (95% CI 67.2-86.3) and prevalence 24.8% (81/326). Discordant outcomes between the 2 sorts of specimens confirmed a big affiliation with low viral load within the pharynx of adults however not of pediatric contributors.

Interpretation: Within the context of a pediatric tertiary care hospital, the sensibility of the take a look at in saliva just isn’t excessive sufficient to switch the use of NP/OP swab for COVID-19 analysis. Neither NP/OP swab nor saliva might detect all of the contributors contaminated with SARS-CoV-2.

Développement d’outils moléculaires pour le diagnostic de la maladie d’Alzheimer basés sur la détection de protéines amyloïdogènes 

Alzheimer’s illness (AD) is the most typical type of dementia that normally happens amongst older folks. AD outcomes from neuronal degeneration that results in the cognitive impairment and demise. AD is incurable, sometimes develops over the course of a few years and is accompanied by a lack of purposeful autonomy, making a affected person utterly depending on relations and/or healthcare staff. Essential options of AD are pathological polymerization of Aβ peptide and microtubule-associated protein tau, accompanied by alterations of their conformations and leading to accumulation of cross-β fibrils (amyloids) in human brains. AD apparently progresses asymptomatically for years and even many years earlier than the looks of signs. Due to this fact, improvement of the early AD analysis at a pre-symptomatic stage is important for potential therapies.

This evaluation is concentrated on present and potential molecular instruments (together with non-invasive strategies) which might be based mostly on detection of amyloidogenic proteins and will be relevant to early analysis of AD.Abbreviations: Aβ – amyloid-β peptide; AβO – amyloid-β oligomers; AD – Alzheimer’s illness; ADRDA – Alzheimer’s Illness and Associated Problems Affiliation; APH1 – anterior pharynx faulty 1; APP – amyloid precursor protein; BACE1 – β-site APP-cleaving enzyme 1; BBB – mind blood barrier; CJD – Creutzfeldt-Jakob illness; CRM – licensed reference materials; CSF – cerebrospinal fluid; ELISA – enzyme-linked immunosorbent assay; FGD – 18F-fluorodesoxyglucose (2-deoxy-2-[18F]fluoro-D-glucose); IP-MS – immunoprecipitation-mass spectrometry assay; MCI – delicate cognitive impairment; MDS – multimer detection system; MRI – magnetic resonance imaging; NIA-AA – Nationwide Institute on Ageing and Alzheimer’s Affiliation; NINCDS – Nationwide Institute of Neurological and Communicative Problems and Stroke; PEN2 – presenilin enhancer 2; PET – positron emission tomography; PiB – Pittsburgh Compound B; PiB-SUVR – PIB standardized uptake worth ratio; PMCA – Protein Misfolding Biking Amplification; PrP – Prion Protein; P-tau – hyperphosphorylated tau protein; RMP – reference measurement process; RT-QuIC – real-time quaking-induced conversion; SiMoA – single-molecule array; ThT – thioflavin T; TSEs – Transmissible Spongiform Encephslopathies; T-tau – whole tau protein.

Laisser un commentaire

Votre adresse e-mail ne sera pas publiée. Les champs obligatoires sont indiqués avec *